Liu Lab Clemson

Publications

algorithm theory software instrumentation application review

Highlighted

Universal inverse modeling of point spread functions for SMLM localization and microscope characterization
Universal inverse modeling of point spread functions for SMLM localization and microscope characterization
Sheng Liu, Jianwei Chen, Jonas Hellgoth, Lucas-Raphael Müller, Boris Ferdman, …, Keith A. Lidke, Rainer Heintzmann, Yoav Shechtman, Yiming Li, Jonas Ries
Nature Methods  ·  01 Jun 2024  ·  doi:10.1038/s41592-024-02282-x
software

All

2025

Enhanced supercritical angle localization microscopy through point spread function modeling
Enhanced supercritical angle localization microscopy through point spread function modeling
Sajjad A. Khan, Keith A. Lidke, Sheng Liu
Biomedical Optics Express  ·  10 Jul 2025  ·  doi:10.1364/BOE.563592
algorithm
Upsampled PSF enables high accuracy 3D superresolution imaging with sparse sampling rate
Upsampled PSF enables high accuracy 3D superresolution imaging with sparse sampling rate
Jianwei Chen, Wei Shi, Jianzheng Feng, Jianlin Wang, Sheng Liu, Yiming Li
Photonics Research  ·  16 May 2025  ·  doi:10.1364/PRJ.547778
algorithm
A MATLAB-based Instrument Control MIC package for fluorescence imaging
A MATLAB-based Instrument Control (MIC) package for fluorescence imaging
Sajjad A. Khan, Sandeep Pallikkuth, David J. Schodt, Marjolein B. M. Meddens, Hanieh Mazloom-Farsibaf, …, Sheng Liu, Ellyse Taylor, Mohamadreza Fazel, Farzin Farzam, Keith A. Lidke
Journal of Open Source Software  ·  28 Jan 2025  ·  doi:10.21105/joss.07275
software
Separation estimation of two freely rotating dipole emitters near the quantum limit
Separation estimation of two freely rotating dipole emitters near the quantum limit
Sheng Liu, Sujeet Pani, Sajjad A. Khan, Francisco E. Becerra, Keith A. Lidke
Cold Spring Harbor Laboratory  ·  03 Jan 2025  ·  doi:10.1101/2025.01.03.631147
theory

2024

Fast and universal single-molecule localization using multi-dimensional point spread functions
Fast and universal single-molecule localization using multi-dimensional point spread functions
Mengfan Li, Wei Shi, Sheng Liu, Shuang Fu, Yue Fei, Lulu Zhou, Ke Fang, Yiming Li
Optics Express  ·  06 Nov 2024  ·  doi:10.1364/OE.531588
algorithm
Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy
Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy
Hassan Dibaji, Ali Kazemi Nasaban Shotorban, Rachel M. Grattan, Shayna Lucero, David J. Schodt, Keith A. Lidke, Jonathan Petruccelli, Diane S. Lidke, Sheng Liu, Tonmoy Chakraborty
Nature Communications  ·  12 Jun 2024  ·  doi:10.1038/s41467-024-49291-0
instrumentation
Universal inverse modeling of point spread functions for SMLM localization and microscope characterization
Universal inverse modeling of point spread functions for SMLM localization and microscope characterization
Sheng Liu, Jianwei Chen, Jonas Hellgoth, Lucas-Raphael Müller, Boris Ferdman, …, Keith A. Lidke, Rainer Heintzmann, Yoav Shechtman, Yiming Li, Jonas Ries
Nature Methods  ·  01 Jun 2024  ·  doi:10.1038/s41592-024-02282-x
software
Signal improved ultra-fast light-sheet microscope for large tissue imaging
Signal improved ultra-fast light-sheet microscope for large tissue imaging
Md Nasful Huda Prince, Benjamin Garcia, Cory Henn, Yating Yi, Etsuo A. Susaki, …, Keith A. Lidke, Hu Zhao, Irene Salinas Remiro, Sheng Liu, Tonmoy Chakraborty
Communications Engineering  ·  02 Apr 2024  ·  doi:10.1038/s44172-024-00205-4
instrumentation

2023

Direct observation of motor protein stepping in living cells using MINFLUX
Direct observation of motor protein stepping in living cells using MINFLUX
Takahiro Deguchi, Malina K. Iwanski, Eva-Maria Schentarra, Christopher Heidebrecht, Lisa Schmidt, …, Sheng Liu, Veronika Chevyreva, Kyung-Min Noh, Lukas C. Kapitein, Jonas Ries
Science  ·  10 Mar 2023  ·  doi:10.1126/science.ade2676
application

2022

Automated multi-target super-resolution microscopy with trust regions
Automated multi-target super-resolution microscopy with trust regions
David J. Schodt, Farzin Farzam, Sheng Liu, Keith A. Lidke
Biomedical Optics Express  ·  20 Dec 2022  ·  doi:10.1364/BOE.477501
algorithm instrumentation
Super-Resolution Microscopy for Structural Cell Biology
Super-Resolution Microscopy for Structural Cell Biology
Sheng Liu, Philipp Hoess, Jonas Ries
Annual Review of Biophysics  ·  09 May 2022  ·  doi:10.1146/annurev-biophys-102521-112912
review

2020

Variance lower bound on fluorescence microscopy image denoising
Variance lower bound on fluorescence microscopy image denoising
Yilun Li, Sheng Liu, Fang Huang
Biomedical Optics Express  ·  09 Nov 2020  ·  doi:10.1364/BOE.401836
theory
Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens
Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens
Sheng Liu, Hyun Huh, Sang-Hyuk Lee, Fang Huang
Annual Review of Biomedical Engineering  ·  04 Jun 2020  ·  doi:10.1146/annurev-bioeng-060418-052203
review
Enhanced 4Pi single-molecule localization microscopy with coherent pupil based localization
Enhanced 4Pi single-molecule localization microscopy with coherent pupil based localization
Sheng Liu, Fang Huang
Communications Biology  ·  08 May 2020  ·  doi:10.1038/s42003-020-0908-2
algorithm
Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval
Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval
Fan Xu, Donghan Ma, Kathryn P. MacPherson, Sheng Liu, Ye Bu, …, Alexander A. Chubykin, Peng Yin, Sarah Calve, Gary E. Landreth, Fang Huang
Nature Methods  ·  01 May 2020  ·  doi:10.1038/s41592-020-0816-x
algorithm

2018

Analyzing complex single-molecule emission patterns with deep learning
Analyzing complex single-molecule emission patterns with deep learning
Peiyi Zhang, Sheng Liu, Abhishek Chaurasia, Donghan Ma, Michael J. Mlodzianoski, Eugenio Culurciello, Fang Huang
Nature Methods  ·  30 Oct 2018  ·  doi:10.1038/s41592-018-0153-5
algorithm
Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections
Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections
Michael J. Mlodzianoski, Paul J. Cheng-Hathaway, Shane M. Bemiller, Tyler J. McCray, Sheng Liu, David A. Miller, Bruce T. Lamb, Gary E. Landreth, Fang Huang
Nature Methods  ·  16 Jul 2018  ·  doi:10.1038/s41592-018-0053-8
instrumentation

2017

sCMOS noise-correction algorithm for microscopy images
sCMOS noise-correction algorithm for microscopy images
Sheng Liu, Michael J Mlodzianoski, Zhenhua Hu, Yuan Ren, Kristi McElmurry, Daniel M Suter, Fang Huang
Nature Methods  ·  01 Aug 2017  ·  doi:10.1038/nmeth.4379
software

2016

Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution
Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution
Marjolein B. M. Meddens, Sheng Liu, Patrick S. Finnegan, Thayne L. Edwards, Conrad D. James, Keith A. Lidke
Biomedical Optics Express  ·  17 May 2016  ·  doi:10.1364/BOE.7.002219
instrumentation

2015

Sequential Superresolution Imaging of Multiple Targets Using a Single Fluorophore
Sequential Superresolution Imaging of Multiple Targets Using a Single Fluorophore
Christopher C. Valley, Sheng Liu, Diane S. Lidke, Keith A. Lidke
PLOS ONE  ·  10 Apr 2015  ·  doi:10.1371/journal.pone.0123941
instrumentation
Resolution improvement by 3D particle averaging in localization microscopy
Resolution improvement by 3D particle averaging in localization microscopy
Jordi Broeken, Hannah Johnson, Diane S Lidke, Sheng Liu, Robert P J Nieuwenhuizen, Sjoerd Stallinga, Keith A Lidke, Bernd Rieger
Methods and Applications in Fluorescence  ·  16 Jan 2015  ·  doi:10.1088/2050-6120/3/1/014003
algorithm

2013

A Multiemitter Localization Comparison of 3D Superresolution Imaging Modalities
A Multiemitter Localization Comparison of 3D Superresolution Imaging Modalities
Sheng Liu, Keith A. Lidke
ChemPhysChem  ·  26 Nov 2013  ·  doi:10.1002/cphc.201300758
theory
Three dimensional single molecule localization using a phase retrieved pupil function
Three dimensional single molecule localization using a phase retrieved pupil function
Sheng Liu, Emil B. Kromann, Wesley D. Krueger, Joerg Bewersdorf, Keith A. Lidke
Optics Express  ·  21 Nov 2013  ·  doi:10.1364/OE.21.029462
algorithm
Multi-Color Quantum Dot Tracking Using a High-Speed Hyperspectral Line-Scanning Microscope
Multi-Color Quantum Dot Tracking Using a High-Speed Hyperspectral Line-Scanning Microscope
Patrick J. Cutler, Michael D. Malik, Sheng Liu, Jason M. Byars, Diane S. Lidke, Keith A. Lidke
PLoS ONE  ·  22 May 2013  ·  doi:10.1371/journal.pone.0064320
instrumentation