Research Projects
Current Projects
MINFLUX
MINFLUX (minimum photon flux) employs a donut shaped laser beam to track the emitter with the local minimum of its illumination pattern. The central minimum of a donut-shaped beam is scanned around the single emitter following a predefined scanning pattern. The photon values detected at all scanning positions, together with the knowledge of the scanning pattern, allow one to extract the emitter’s position with nanometer precision. In single-particle tracking, the donut beam continuously follows the emitter with a localization rate up to the scanning cycle rate of approximately 10 kHz. Our lab is interested in constructing a custom-built MINFLUX system and applying it to studying protein dynamics on cells.
Direct observation of motor protein stepping in living cells using MINFLUX
Science
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10 Mar 2023
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doi:10.1126/science.ade2676
4Pi-SMLM
4Pi-SMLM
Universal inverse modeling of point spread functions for SMLM localization and microscope characterization
Nature Methods
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01 Jun 2024
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doi:10.1038/s41592-024-02282-x
Enhanced 4Pi single-molecule localization microscopy with coherent pupil based localization
Communications Biology
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08 May 2020
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doi:10.1038/s42003-020-0908-2
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